Can propidium iodide stain live cells?
Can propidium iodide stain live cells?
Propidium iodide is a vital stain. a live cells exclude the dye and cannot be stained. It stains only dead cells. It will also be dependant on the concentration of PI that you are using.
Does propidium iodide stain DNA?
Propidium iodide is a membrane-impermeable fluorescent DNA stain. It is used to stain apoptotic cells for flow cytometry, in situ hybridization, and immunohistochemistry applications Belloc et al (1994). It can also be used to differentiate between apoptotic and necrotic cell death.
Is propidium iodide water soluble?
Solubility Data for Propidium iodide
| Solvent | Max Conc. mg/mL | |
|---|---|---|
| Solubility | ||
| water | 3.34 |
How do you dissolve propidium iodide?
To make a stock solution from the solid form, dissolve PI (MW = 668.4) in deionized water (dH2O) at 1 mg/mL (1.5 mM) and store at 4°C, protected from light. When handled properly, solutions are stable for at least 6 months.
What is propidium iodide used for?
Propidium iodide (PI) is a popular red-fluorescent nuclear and chromosome counterstain. Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population. PI binds to DNA by intercalating between the bases with little or no sequence preference.
Why does propidium iodide staining indicate apoptosis?
Annexin V and PI are double-staining probes for apoptosis by detecting the externalization of phosphatidylserine and membrane integrity. The apoptotic cells externalize their phosphatidylserine early in apoptosis when the cell membrane is still intact.
Is propidium iodide cell permeable?
Membranes of stressed Saccharomyces cerevisiae are permeable to propidium iodide.
What is the spectrum of propidium iodide?
Propidium Iodide Spectrum. Molecular Structure of Propidium Iodide. Diagram depicts the skeletal structure of propidium iodide, chemical formula C 27 H 34 I 2 N 4. Propidium Iodide (PI) binds nucleic acids by intercalation in the bases. When bound to nucleic acid it has an excitation maxima ~535 nm and an emission maxima ~615 nm.
How does propidium iodide detect dead cells?
Propidium iodide (PI) is a popular red-fluorescent nuclear and chromosome counterstain. Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population. PI binds to DNA by intercalating between the bases with little or no sequence preference.
What is the excitation and emission maxima of propidium iodide?
Propidium Iodide (PI) binds nucleic acids by intercalation in the bases. When bound to nucleic acid it has an excitation maxima ~535 nm and an emission maxima ~615 nm. On a flow cytometer PI is typically excited by 488 or 561 nm and can be detected in a 610/20 bandpass.
How does propidium iodide (PI) bind nucleic acids?
Propidium Iodide (PI) binds nucleic acids by intercalation in the bases. When bound to nucleic acid it has an excitation maxima ~535 nm and an emission maxima ~615 nm.