What are L929 cells?
NCTC clone 929 [L cell, L-929, derivative of Strain L] (connective mouse tissue) is the first clone of strain L, one of the first cell strains to be established in continuous culture. Use NCTC clone 929 for cell line toxicity testing and as a transfection host.
How is bone marrow derived from macrophages?
Bone marrow-derived macrophages (BMDM) are primary macrophages obtained by in vitro differentiation of bone marrow cells in the presence of macrophage colony-stimulating factor (M-CSF or CSF1). They are easy to obtain in high yields, can be stored by freezing, and can be obtained from genetically modified mice strains.
How do you make a L929 conditioned medium?
Preparation of L929-Conditioned Medium
- Plate 4.7 × 105 L929 cells in a 75-cm2 flask containing 55 mL of L929 medium.
- Grow cells in a humidified incubator with 5% CO2 at 37°C for 7 d.
- Collect the supernatant. Filter through a 0.45-μm filter. Store 50-mL aliquots frozen at −20°C (L929-conditioned medium).
What cells differentiate into macrophages?
Monocytes can differentiate into inflammatory or anti-inflammatory subsets. Upon tissue damage or infection, monocytes are rapidly recruited to the tissue, where they can differentiate into tissue macrophages or dendritic cells.
Are L929 cells adherent?
The L929 cells exhibited adherent growth and heterogeneous in morphology, including spindle-like, epithelial-like, stellate, and round shape (Figure 1a).
Are osteoblasts related to macrophages?
Close to the bone surface, osteal macrophages are adjacent to osteoblasts, regulate bone formation, and are closely related to the osteogenic differentiation of mesenchymal stem cells.
Are there macrophages in the bone marrow?
Macrophages in the bone marrow play an important role in hematopoiesis by providing signals that induce differentiation and proliferation of the earliest committed erythroid progenitors. Macrophages are thus important for RBCs throughout their lifespan.
What are peritoneal macrophages?
Peritoneal macrophages are the macrophages that reside in the peritoneal cavity, a fluid-filled space located between the wall of the abdomen and the organs found in the abdomen. In the absence of peritoneal infection or inflammation, peritoneal macrophages are thought to have anti-inflammatory functions.
Which cells differentiate into macrophages quizlet?
* Monocytes differentiate into Macrophages. * B Lymphocytes differentiate into Plasma Cells. Differentiate into Reticulocytes, Megakaryocytes, Eosinophils, Basophils, Neutrophils, Monocytes, and Mast Cells. You just studied 7 terms!
How do monocytes perform amoeboid movement and phagocytosis?
Monocytes can perform phagocytosis. They do this by using intermediary or opsonising proteins such as antibodies or complement that coat the pathogen. In this way they perform amoeboid movement and indulge in phagocytosis.
What is the origin of L929 cells?
L929 is a fibroblast cell line derived from a clone of normal subcutaneous areolar and adipose tissue of a male C3H/An mouse (9, 10). It was found to secrete a macrophage growth factor (11), which was later identified as M-CSF (4).
Are L929-and M-CSF-derived macrophages the same?
Macrophages can be derived from the bone marrow of mice with either recombinant M-CSF or L929 supernatant. Recent literature considers recombinant M-CSF- and L929-derived macrophages as equals, even though L929-derived macrophages are exposed to other substances secreted in the L929 supernatant, and not only M-CSF.
How does the composition of L929 cell-conditioned media affect BMDM phenotype?
Commonly used methods to differentiate macrophages from BM are treatment with either recombinant M-CSF or the supernatant of L929 cells, which secrete M-CSF. However, little is known about the composition of L929 cell-conditioned media (LCCM) and how it affects the BMDM phenotype.
How to prepare GM-CSF from L929 cells?
L929 cells produce GM-CSF. At confluence, replace culture media with fresh complete DMEM. Transfer flasks to 32 °C, 5% CO 2 for 10 days. Collect, pool and centrifuge the supernatants at 750 x g for 10 min. Discard cell pellets. Store supernatants in 15 ml tubes and store at -20 °C.