What is LR recombination?

What is LR recombination?

The LR Reaction, again is a recombination reaction between attL and attR sites. The reaction generates an expression clone and is catalyzed by recombinant proteins. Recombination between these sites generates two molecules. One molecule contains the DNA segment of interest, the other molecule is a by-product.

What is pENTR d TOPO?

The pENTR™/D-TOPO® Cloning Kits utilize a highly efficient, 5-minute cloning strategy (“TOPO® Cloning”) to directionally clone a blunt-end PCR product into a vector for entry into the Gateway® System or the MultiSite Gateway® System.

What are entry clones?

An Entry clone contains your gene of interest flanked by attL sequences, which are then used to recombine with attR sequences to create your desired expression clone.

What is a destination vector?

Gateway Destination vectors are similar to classical expression vectors containing multiple cloning sites, before the insertion of a gene of interest, using restriction enzyme digestion and ligation. Gateway Destination vectors are commercially available from Invitrogen, EMD (Novagen) and Covalys.

What is Gateway Destination?

A tourist gateway (sometimes called a “Tourism Gateway” or “Gateway City”) is a place or settlement through which tourists typically first visit on their way to a tourist attraction or tourism region. Sometimes these services can be in direct competition with those offered at the tourist attractions themselves.

What is a gateway vector?

Gateway vectors contain modified versions of the att sites so that scientists can easily clone in their desired DNA sequences.

What is the efficiency of the pdonr221 vector?

The PCR product is directionally cloned with efficiencies typically >99%. The pDONR™221 vector has a pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use. For Research Use Only. Not for use in diagnostic procedures. 6 µg pDONR221™ vector, lyophilized in TE buffer, pH 8.0. Upon receipt, store at -20°C.

What is Invitrogen?

Invitrogen creates products for cellular, protein and molecular biology research that allow scientists to innovate. Shop Invitrogen products at Fisher Scientific. Invitrogen | Fisher Scientific Fisher Scientific Fisher Healthcare Fisher Science Education Sign Up for Email Help & Support Center › 0 No offer available

What is Invitrogen 10 mm dNTP mix?

Invitrogen 10 mM dNTP Mix is a mixture of four nucleotides (dATP, dCTP, dGTP, dTTP) in 0.6 mM Tris-HCl. 12 Invitrogen™ EVOS™ XL Core Configured Cell Imager The EVOS XL Core Imaging System is a digital transmitted-light inverted imaging system for cell and tissue culture applications and routine cell maintenance.

What is Invitrogen™ Dynabeads?

Invitrogen™ Dynabeads™ MyOne™ Streptavidin C1 Exceptional standard for isolation and handling of biotinylated nucleic acids, antibodies, or other biotinylated ligands and targets Sheep IgG (H+L) Cross-Adsorbed Donkey anti-Ovine, Alexa Fluor™ 647, Invitrogen™ Donkey Polyclonal Secondary Antibody