What is the function of fimB?
It was found that fimB mediates DNA inversion from on to off, as well as from off to on, and that, contrary to prior interpretations, the fimB-associated inversion occurs with only minimal orientational preference to the on phase.
What is mucoid E coli?
Mucoid strains of E. coli produce extracellular slime consisting either of a polysaccharide of certain K antigen specificities, or a common acid polysaccharide (often described as M antigen) composed of colanic acid (Jiménez et al., 2012).
What is the sequence of E coli?
The nucleotide sequence of the Escherichia coli glyA gene has been determined. The amino acid sequence predicted from the DNA sequence consists of 417 residues. After the coding region there is a 185 nucleotide sequence preceding the proposed transcription termination region for the glyA gene.
Does Escherichia coli stain well?
Most E coli strains are nonpigmented. The image below shows Escherichia coli on Gram staining.
Is E. coli a mucoid colony?
Those who work with Escherichia coli and other Gram- negative bacteria occasionally see glossy, raised and opaque, ‘mucoid’ colonies on agar that can be attributed to contamina- tion with bacteriophage.
Has E. coli been sequenced?
In September 1997, the complete genome sequence of Escherichia coli was published. E.
What is the size of E. coli?
Escherichia coli is a typical gram-negative rod bacterium. Its dimensions are those of a cylinder 1.0-2.0 micrometers long, with radius about 0.5 micrometers.
Which bacteria contain fimbriae?
Fimbriae are one of the primary mechanisms of virulence for E. coli, Bordetella pertussis, Staphylococcus and Streptococcus bacteria. Their presence greatly enhances the bacteria’s ability to attach to the host and cause disease.
How does fimbriae help bacteria cause disease?
Fimbriae facilitate adherence and thus enhance the capacity of the organism to produce disease. E coli, P mirabilis, and other gram-negative bacteria contain fimbriae (ie, pili), which are tiny projections on the surface of the bacterium.
Is E. coli lactose fermenting?
E. coli are facultative anaerobic, Gram-negative bacilli that will ferment lactose to produce hydrogen sulfide. Up to 10% of isolates have historically been reported to be slow or non-lactose fermenting, though clinical differences are unknown.
What does E. coli bacteria look like?
E. coli is a rod-shaped bacterium of the Enterobacteriaceae family. It can live in environments with or without air. These bacteria live in the intestines of healthy people and warm-blooded animals.
What is the function of FimB in E coli?
FimB is one of the 2 regulatory proteins which control the phase variation of type 1 fimbriae in E.coli. These proteins mediate the periodic inversion of a 300bp DNA segment that harbors the promoter for the fimbrial structural gene, FimA. FimB switches FimA on.
What is the FIM recombinase in Escherichia coli?
The phase variation of type 1 fimbriae in Escherichia coli is associated with the site-specific inversion of a short DNA element. Recombination at fim requires fimB and fimE, and their products are considered to be the fim recombinases.
Which regulatory FIM genes control phase variation in Escherichia coli type 1 fimbriae?
Klemm, P. 1986. Two regulatory fim genes, fimB and fimE, control the phase variation of type 1 fimbriae in Escherichia coli. EMBO J. 5:1389-1393
Which FIM genes are responsible for synthesis of Type 1 fimbriae?
The fim genes responsible for synthesis of type 1 fimbriae in Escherichia coli, cloning and genetic organization. Mol.Gen.Genet. 199:410-414