What is the wavelength of mCherry?
mCherry absorbs light between 540-590 nm and emits light in the range of 550-650 nm. mCherry belongs to the group of fluorescent protein chromophores used as instruments to visualize genes and analyze their functions in experiments.
What color is mCherry?
mCherry is a bright red monomeric fluorescent protein created by rounds of directed evolution of DsRed.
At what wavelength does YFP fluorescence?
In the Nikon YFP HYQ block, excitation is limited to a 20-nanometer band of wavelengths in the blue-green spectral region (490 to 510 nanometers), and is coupled to an emission filter transmitting yellowish-green fluorescence in the range of 520 to 550 nanometers.
What wavelength does YFP absorb?
The visible light absorption maximum of fluorescein is 490 nanometers and the emission maximum occurs at 525 nanometers.
What is the difference between TagRFP and mCherry spectra?
Here, the TagRFP spectrum is shown in the darker colors and the mCherry spectrum is shown in the lighter colors; excitation spectra are blue and emission spectra are red.
What is TagRFP?
TagRFP is a basic (constitutively fluorescent) red fluorescent protein published in 2007, derived from Entacmaea quadricolor. It has very low acid sensitivity.
What is the origin of the red fluorescent protein mCherry?
mCherry is a basic (constitutively fluorescent) red fluorescent protein published in 2004, derived from Discosoma sp.. It is reported to be a very rapidly-maturing monomer with low acid sensitivity. DsRed.
What are the wavelengths of the laser lines shown?
The wavelengths of the laser lines, 405, 458, and 488 nm, are shown by vertical lines with indication of excitation efficiency. The emission that passes through the 550 nm long pass (red dash line) and 640/50 nm band pass (blue dash line) filters is shown as cross-hatched region with square S1and S2, respectively.