What is XK column?
What is XK column?
XK empty columns are designed for liquid chromatography at low to medium pressure. XK columns are compatible with aqueous solutions and most organic solvents used in liquid chromatography of macromolecules.
What is a chromatography column made of?
Materials. Liquid chromatography: Traditional chromatography columns were made of glass. Modern columns are mostly made of borosilicate glass, acrylic glass or stainless steel. To prevent the stationary phase from leaking out of the column interior a polymer, stainless steel or ceramic net is usually applied.
What is slurry in column chromatography?
For the wet method, a slurry is prepared of the eluent with the stationary phase powder and then carefully poured into the column. During the entire chromatography process the eluent is collected in a series of fractions. Fractions can be collected automatically by means of fraction collectors.
What is BPG column?
BPG columns are glass chromatography columns designed for industrial applications, which demand high standards of hygiene. The columns are constructed from component materials of the highest quality and withstand the harsh conditions used for cleaning in place of packed downstream purification resins.
What are the two methods of packing a chromatography column?
There exists two methods to fill columns: • dry packing, • slurry packing (filtration technique). The dry filling method is easy to perform, but the slurry method gives, especially for smaller particles, more efficient and more reproducible columns.
What is mean column volume?
The column volume is the volume of the entire column. Since the column is cylindrical, the formula for the volume of a cylinder is used in calculations. The bed volume refers to the volume of the adsorbent packing within the column. Typically, the bed volume should be lesser than the column volume.
What is CV in column chromatography?
Column Volume (CV) is a term commonly used with flash chromatography to describe compound retention or the amount of volume of mobile phase required to elute a compound. A specific compound’s CV is equal to the reciprocal of its Rf (1/Rf).
How do you prevent air bubbles in column chromatography?
Prevent large air bubbles from being drawn down into a column resin bed by always removing the column’s top cap before the bottom cap and replacing the bottom cap before the top cap.
What are the dimensions of the XK 26 and hiscale 50?
XK 26 and HiScale 26 185.7 31 1.09 31 350 XK 50 and HiScale 50 686.9 115 1.09 115 1 Part of the CDM program from Cytiva.
How many XK adapters come with the XK column?
* Each XK column is delivered with one XK adapter and one bottom piece. Sample applicators Code No. Manual valves Code No. Superloop™ 10 ml, 1/16” fitting 18-1113-81 SRV-3 19-2098-01 Superloop 50 ml, 1/16” fitting 18-1113-82 SRV-4 19-2099-01 V-7 19-7500-01 V-8 19-7576-01 Union 5/16 Fe HPLC 18-1142-08
What is the length of an Xk?
Length (cm) i.d. 16 mm i.d. 26 mm i.d. 50 mm 20 XK 16/20 XK 26/20 XK 50/20 3–X 0 K 50/30 40 XK 16/40 XK 26/40 – 6–X 0 K 50/60 70 XK 16/70 XK 26/70 – 100 XK 16/100 XK 26/100 XK 50/100 2 3 4 5 1 6 3 2 7 4 8
What is the maximum temperature range for the xk-16 column?
The column may be used at temperatures up to 40°C and pressures up to 0.5 MPa (5 bar) for XK 16 and XK 26 columns, and 0.3 MPa (3 bar) for XK 50 columns. The jacket can also withstand temperatures up to 40°C but should not be exposed to pressures higher than 0.2 MPa (2 bar).