How do you make an X-Gal stock solution?

Make a 2% (w/v) stock solution by dissolving X-gal in dimethylformamide at a concentration of 20 mg/ml solution. Use a glass or polypropylene tube. Wrap the tube containing the solution in aluminum foil to prevent damage by light and store at -20°C. It is not necessary to sterilize X-gal solutions by filtration.

What is the function of X-Gal?

X-Gal is a mainstay in molecular biology cloning applications in which it is used to detect the activity of β-galactosidase. X-Gal is used to detect the insertion of foreign DNA into the lacZ region of a plasmid DNA. Insertion of DNA into the lacZ region results in the loss of β-galactosidase activity.

Can you dissolve X-Gal DMSO?

X-gal is soluble in organic solvents such as DMSO and dimethyl formamide. The solubility of X-gal in these solvents is approximately 30 mg/ml.

What is the function of X-Gal and IPTG?

X-Gal (5-Bromo-4-Chloro-3-Indolyl-beta-D-Galactoside) is a chromogenic substrate for beta-galactosidase that yields a blue precipitate upon hydrolysis, while IPTG (isopropyl beta-D-1-thiogalactopyranoside) induces the transcription of genes from the lac and tac operons in bacteria, notably the hydrolase enzyme beta- …

What type of protein is B galactosidase?

β-Galactosidases 33 and encodes a 677-amino-acid protein. The enzyme is more precisely referred to as GM1 ganglioside β-galactosidase and deficiencies are associated with GM1 gangliosidosis. GM1 ganglioside β-galactosidase has an acidic pH optimum. It is activated by chloride ions.

Why do bacteria perform transformation?

Bacterial transformation is a key step in molecular cloning, the goal of which is to produce multiple copies of a recombinant DNA molecule. Prior steps for creating recombinant plasmids are described in traditional cloning basics and involve insertion of a DNA sequence of interest into a vector backbone.

Is DMSO safe for human use?

When applied to the skin: Non-prescription DMSO is POSSIBLY UNSAFE. Some non-prescription DMSO products might be “industrial grade,” which is not intended for human use. These products can contain impurities that can cause health issues.

How do you make X gal solution?

X-gal solution. X-gal is 5-bromo-4-chloro-3-indolyl-β-D-galactoside. Make a 2% (w/v) stock solution by dissolving X-gal in dimethylformamide at a concentration of 20 mg/ml solution. Use a glass or polypropylene tube. Wrap the tube containing the solution in aluminum foil to prevent damage by light and store at -20°C.

What is the size of the XFX gal solution?

X-Gal Solution, ready-to-use, is stable, 0.22 µm membrane filtered solution formulated for direct use in conjunction with IPTG for blue/white colony screening. For Research Use Only. Not for use in diagnostic procedures. Convenient, on-site access to the products you need.

What is the concentration of X-gal?

X-gal is 5-bromo-4-chloro-3-indolyl-β-D-galactoside. Make a 2% (w/v) stock solution by dissolving X-gal in dimethylformamide at a concentration of 20 mg/ml solution.

What is the storage condition for X gal?

X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype. Storage conditions (working solution): X-gal in solution (i.e., dissolved in DMF 2% (w/v)) is stable for several weeks when stored in the dark at 2 to 8 °C; when the solution is stored at -15 to -25 °C, it is stable for one year.