What is P Akt Ser473?
Phospho-Akt (Ser473) Antibody detects endogenous levels of Akt1 only when phosphorylated at Ser473. This antibody also recognizes Akt2 and Akt3 when phosphorylated at the corresponding residues.
What is phospho Akt?
The serine/threonine protein kinase AKT is a key player in the regulation of cell survival. AKT functions to promote cell survival by inhibiting apoptosis through its ability to phosphorylate and inactivate several targets including Bad, caspase-9 and Forkhead transcription factor.
What is Thr308?
Abbreviations: Ser473=serine 473; Thr308=threonine 308.
Where does mTORC2 phosphorylate Akt?
It is clear from the foregone discussion that there is a reciprocal relation between Akt and the two mTOR complexes. IIS mediated phosphorylation of Akt-T308 activates mTORC1, and mTORC2 phosphorylates Akt on S473.
What is cleaved caspase-3?
The cleaved-Caspase-3 (Asp175) antibody (referred to as cleaved-Caspase-3 antibody) from Cell Signaling Technology (Danvas, MA, USA) is a polyclonal antibody obtained from rabbit that was raised against a peptide in the large subunit of the human effector caspase, Caspase-3, amino-terminal to Asp175.
Who phosphorylates Akt?
AKT is also phosphorylated at the hydrophobic motif, S473, by TORC2 (target of rapamycin complex 2) and DNA-PK (DNA-activated protein kinase) [11, 17, 19, 20] and possibly by rapamycin-insensitive TORC1 .
What is the molecular weight of Akt in kDa?
Molecular Weight AKT – 56 kDa Molecular Weight phospho-AKT – 60 kDa (Figure 1) Figure 1. WB: HEK-293 cell lysate was subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT antibody) and 66444-1-Ig (AKT-phospho-S473 antibody) at a dilution of 1:4000 incubated at room temperature for 1.5 hours.
What is the function of PI3K in Akt?
An important function of activated PI3K in cells is the inhibition of programmed cell death ( Yao and Cooper, 1995 ), and Akt is a good candidate for mediating these PI3K-dependent cell-survival responses.
What is AKT (AKT pathway)?
1) What is AKT? The serine/threonine kinase B AKT pathway (also known as the PI3K-Akt pathway) plays a vital role in the regulation of cellular processes, including cell proliferation, survival, and growth – processes that are essential for oncogenesis.
How many isoforms of Akt are there?
In mammals, three closely related isoforms of Akt are encoded by distinct genetic loci: Akt (that in fact is Akt1), Akt2 and Akt3.