What is 2 Chlorotrityl chloride resin?

What is 2 Chlorotrityl chloride resin?

2-Chlorotrityl chloride resin can be cleaved under extremely mild acid conditions that leave Boc/tBu based protecting groups in place. Thus it is used to prepare protected peptide fragments. Amino acids can be attached to 2-Cl-Trt chloride resin with very little or no racemization.

How do you Deprotect FMOC?

The Fmoc group is, in general, rapidly removed by primary (i.e., cyclohexylamine, ethanolamine) and some secondary (i.e., piperidine, piperazine) amines, and slowly removed by tertiary (i.e., triethylamine [Et3N], N, N-diisopropylethylamine [DIEA]) amines.

How do you cleave Wang resin?

Wang resin is the standard peptide synthesis resin used with Fmoc-chemistry. The resin is acid labile and finished peptides can be easily cleaved by treatment with 50 % (v/v) TFA/DCM. These relatively mild cleavage conditions have made this resin popular also in solid phase organic synthesis.

How do you load Wang resin?

Attachment of the first amino acid on Wang resin

  1. Swell the resin in the mixture of DCM and DMF (9:1, v/v; 10 mL/g).
  2. Dissolve completely 4 eq (relative to the resin) of the Fmoc-AA and 4 eq of HOBt in a minimum amount of DMF.
  3. Dissolve 0.1 eq of DMAP in minimum volume of DMF.
  4. Add 4 eq of DIC to the resin and agitate.

How do you calculate theoretical yield in peptide synthesis?

For example, let’s assume your peptide has a molecular weight of 800 g/mol. And I’m assuming your resin loading capacity is 0.71 mmol/g and you want to use 300 mg of resin. So theoretically you would get: 0.71 mmol/g * 0.3 g * 800 g/mol * 1 mol/1000 mmol = 0.17 g = 100% yield.

How do you cleave Fmoc?

Piperazine/DBU/FA Fmoc Deprotection​

  1. Prepare 5% piperazine : 1% DBU : 1% Formic Acid (FA) : 93 % DMF (w : v : v : v)
  2. Swell the resin in DMF for at least 20 min.
  3. Fill the reaction vessel with at least 2/3 of volume with the cleavage solution.
  4. Stir the reaction mixture for 20 min.
  5. Remove the solution by filtration.

How do you resin a cleave peptide?

Place the peptide resin in a sintered glass funnel and apply some suction. Wash with DMF, acetic acid, then with DCM several times. Wash further with MeOH (polystyrene) or ether (polyacrylamide) to shrink the resin. Remove the peptide resin and dry under a high vacuum for 4 h, or preferably o/n, over KOH.

How do you calculate resin loading?

Substituting the extinction coeffient, volume, dilution and cell width into the general formula results in this formula which can be used to calculate the resin loading. L = (10 x A301)/(7800 x M) in units of mmols/gram.